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Table 3 Coding and scoring scheme used in the study

From: Is the undergraduate microbiology curriculum preparing students for careers in their field?: an assessment of biology majors’ conceptions of growth and control of microorganisms

Coding scheme

Scoring scheme

Question

Category

Sub-category

Score awarded

Suggesting a method for sterilizing:

(a) A solution of vitamins

(b) Contaminated hospital linen

(c) Petri dishes and giving a reason for the method used

(maximum score = 3)

Correct (C)

(a) Correctly identified filtration as the suitable sterilization technique citing the property of vitamins to get denatured upon exposure to heat as the reason for choosing this method.

1

(b) Correctly identified autoclaving as the suitable sterilization technique and considered the bulk of the material to be sterilized.

1

(c) Correctly identified autoclaving or hot air oven as the suitable sterilization technique citing that high temperature would lead to denaturation of the proteins of microorganisms resulting in their death.

1

Partially correct (PC)

Either one of the correct method or a relevant consideration in choosing a method identified

0.5 each for (a), (b), and (c)

Incorrect (IC)

Incorrect method

0

Incorrect method, reason correct

0

Incorrect reason, method correct

0

Unrelated

0

Not attempted (NA)

0

Mathematical problem

(maximum score = 2)

Correct (C)

Identified exponential growth of the organism and calculated the number of cells correctly

2

Partially correct (PC)

Exponential growth of E.coli identified correctly but arithmetical errors made

0.5

Incorrect (IC)

Microbial growth considered to be linear

0

Unrelated (U)

Random calculation with use of unjustifiable and unexplained numbers

0

Not attempted (NA)

0

Doctor’s procedure

(maximum score = 3)

Correct (C)

Burns patient is at a higher risk of infection + technique not sufficient + method of sterilization suggested

1 + 1 + 1

Partially correct (PC)

Any 1 of the above points mentioned

1

Burn patient is at a high risk of infection + technique not sufficient

1 + 1

Burn patient is at a high risk of infection + sterilization needs to be done

1 + 1

Technique used by doctor is not sufficient + sterilization needed

1 + 1

Incorrect (IC)

Doctor followed the correct procedure

0

Unrelated (U)

Random answers unrelated to the question

0

Not attempted (NA)

0