Table 4 The application questions presented to students during Miguel’s instruction

1

You made jam with 0.5 lb of blueberries. These berries were contaminated with 500 endospores of Clostridium botulinum. After 100 cell divisions, how many bacteria would you have in your jam?

2

Suppose 1000 bacteria are inoculated in a tube containing a minimal salts medium, where they double once an hour, and 10 bacteria are inoculated into a rich medium, where they double every 20 min. Which tube will have more bacteria after 2 h? After 4 h?

3

For Application question 2, determine and compare the specific growth rate (k) for these two cultures.